Jnec.org

Dr. Babasaheb Ambedkar Marathwada University, Aurangabad
Proposed Scheme of Instructions and Examination for Fourth Year
(Biotechnology) to be effective from the academic Year 2011 – 2012.
Semester I
Semester II
Plant Tissue Culture and Plant Biotechnology ** Elective – II10.1 Environmental Biotechnology10.2 Animal cell Science and Technology10.3 Food Biotechnology FERMENTATION TECHNOLOGY-II
Lecture: h / week - 4; Theory paper = 100 marks; Duration: 3h Practical: h/week - 2; Term work: 25 marks; Practical Exam: 25 marks UNIT I: Aeration And Agitation
Aeration and agitation with emphasis on determination of kLa, its methods-Aeration and Agitation, effect of shear, O2 requirement of microorganisms, masstransfer theory, diffusional resistance to oxygen transfer, measurement of masstransfer coefficient and factors affecting them. Effect of Aeration and Agitation onmass transfer.
UNIT II: Instrumentation And Control
Methods of measuring process variables, online analysis of chemical factors,control systems viz, manual, automatic and combination, controllers.
UNIT III: Sterilization
Need for sterilization, different types of sterilization techniques – their mechanismof destruction, in situ sterilization, HTST, sterilization indicators, sterilization offermenter, sterilization of media.
UNIT III: Fermentative Production Of Antibiotics
Antibacterial antibiotics: penicillin, streptomycin, chloromycetin, tetracyclines,semisynthetic penicillins; Antifungal antibiotics.
UNIT IV: Fermentative Production Of Amino Acids
UNIT V: Fermentative Production Of Vitamins
Microbial production of vitamins B2 and B12 UNIT VI: Fermentative Production Of Industrially Important Metabolites
Microbial production of industrially important products like ethanol and organicacids; Alcohol Production-wine and other alcoholic beverages, glycerol, beer;organic acids like citric acid, gluconic acid and lactic acid RECOMMENDED BOOKS
1. Fermentation Microbiology and Biotechnology – El-Mansi E.M.T. and 2. Principles of Fermentation Technology – Stanbury P.F. and Whitaker A 3. Industrial microbiology and introduction by Michael J Waites, Neil L.
Morgan, John S. Rockey, Gary Hington, published by Blackwell scienceLtd. London.
4. Fermentation biotechnology: principles, processes and products, Owen P 5. Practical fermentation technology, B. McNeil, L. M. Harvey, John Wiley 6. Bioprocess engineering: basic concepts, by Michael L Schuler and Fikret 7. Microbial Technology, Rajni Gupta and K. G. Mukherjee, Kulbhushan 8. Introduction to Biochemical Engineering, by D. G. Rao, 2nd edition, Tata PRACTICAL
1. Isolation and identification of microbial flora from soil or water sample2. Submerged fermentation of amylase production3. Solid state fermentation for amylase production4. Determination of specific growth rate constant using Monod kinetics5. Whole cell immobilization by entrapment method6. Determination of capacity of resin7. Ammonium salt precipitation of a protein8. Aqueous two phase extraction for a protein PRACTICAL WORK
Note: Any 4 practicals will be conducted from the above list. The practical
examination shall consist of performing an experiment based on the practical work
done during the course, the record of the experiments submitted by the candidate
and viva-voce based on the syllabus.
NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to the question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of a topic.
The question be a full question of 16/18 marks or have sub-questions tomake a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / BIOINFORMATICS
Theory: h/week - 4; Paper: 100 marks; Duration: 3 h Practical: h/week - 2; Practical Examination 50 marks; Term Work: 25 marks Introduction to Bioinformatics, Terminologies used in Bioinformatics, Scope andGoal of Bioinformatics, Overview of applications of Bioinformatics, Databases,Database Management system, Data structure, Database query language,Relational Model, Object Model, Object oriented and Relational databases,Network of databases for Entrez and SRS. Overview of Biological Databases.
DNA sequencing, Nucleotide databases as Genbank, Dna Data Bank of Japan (DDBJ), European Molecular Biology Laboratory (EMBL), NCBI’s Data model,Specialized genomic recourses as SGD, UniGene, TDB. Sequence retrievalsystems such as Entrez and SRS.
Protein structures, Levels of Protein sequence and Structural organization, Proteindatabases, Primary protein sequence Databases as PIR, MIPS, SWISS-PROT,TrEMBL. Composite Protein sequence databases as NRDB, OWL. Secondarydatabases as PROSITE, PRINTS, Blocks. Structure Classification databases asSCOP, CATH, PDB. Various analytical tools for protein Structure Visualization.
Introduction to sequence alignment, Pairwise and Multiple Sequence Alignment,Dot Plot, Needleman Wunsch Algorithm, Smith Waterman Algorithm, Local andGlobal Sequnce Alignment, Substitution Matrices such as PAM And BLOSUM,Calculation of alignment's statistical significance, Importance of Identity matrixes,gaps and penalties, Heuristic methods such as FASTA, Working of FASTA andVariants, BLAST, Working of BLAST and different variants of BLAST.
Introduction to Phylogeny, Homologes, orthologs and paralogs, Construction of aphylogenetic tree, Different types of trees as rooted and unrotted trees.
Phylogenetic analysis, Methods of Phylogenetic analysis as Distance method,Application of bioinformatics in vaccine designs, Drug designing – Ligand basedand Structure based, scoring function for Docking, HTS, QSAR.
Introduction to biotechnology Management, General introduction to managementstudies, Correlation of the management and biotechnology industry, Organizationalmanagement in biotechnology companies, various disciplines in management.
Technology transfer management in Biotechnology.
RECOMMENDED BOOKS
1. Introduction to Bioinformatics, by Arthur M. Lesk Oxford University Press, 2. Bioinformatics- Methods & Applications by S.C.Rastogi, N. Mandiratta, P.
3. Principles of management- Knoots and O.Donnell4. Bioinformatics Sequence and Genome Analysis by David W. Mount, 2nd edition, Cold Spring Harbor Laboratory Press.
5. Bioinformatics: A practical guide to the analysis of genes and proteins A.D.
Baxevanis and B.F.F. Ouellette (Eds). 2002 John Wiley and Sons.
6. Bryan Bergeron, “Bioinformatics computing”, Pearson Education [BB] PRACTICALS
1. Study of Databases and Data retrieval: i. Genome Databasesii. Sequence Databaseiii. Secondary Databases 2. Database Similarity Search BLAST AND FASTA PROGRAMS.
3. PSI/ PHI -BLAST4. Multiple sequences Alignment using ClustalW.
5. Phylogenetic Analysis using Clustalw and Phylip software.
6. Profile Analysis, Motif searching against Motif databases.
7. Rasmol (3D structure visualisation)8. Protein Manipulation using SPDBV9. Introduction to modeller10.Homology modelling using modeller, and swiss model11.UNIX Commands12.Different versions of Linux PRACTICAL WORK
Note- Any 4 practicals will be conducted from the above list. The practical
examination shall consist of performing an experiment based on the practical work
done during the course, the record of the experiments submitted by the candidate
and viva-voce based on the syllabus.
NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to the question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of 04 hrs of a topic. The question be a full question of 16/18 marks or have sub-questionsto make a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / ADVANCE GENETIC ENGINEERING
Lecture: h / week - 4; Theory paper = 100 marks; Duration: 3h UNIT I: Production Of Therapeutic Agents
Engineering human interferon, human growth hormone, r-haematopoeitic growthfactors, r-insulin, interleukins, interferon, r-tissue type plasminogen, r-humandeoxyribonuclease, Enzymes-DNAase I and Alginate lyase against cystic fibrosis;Subunit vaccines-Peptide vaccines, Attenuated vaccines-Vector vaccines.
UNIT II: Synthesis Of Commercial Products
Restriction endonucleases, Small biomolecules, Ascorbic acid, Biopolymers.
UNIT III: Application Of Plant Genetic Engineering
Developing insect-resistance, disease-resistance and herbicide resistance in plantsand delaying fruit ripening; Developing stress and senescence-tolerance in plants,oxidative, salt and submergence stress; Genetic manipulation of flowerpigmentation; Developing quality of seed storage, Provitamin A; Modification offood plant taste and appearance, yield increase in plants; Wild plant relatives as asource of novel genes; Plants as bioreactor - antibodies, polymers, foreign proteinsin seeds; Genome mapping efforts in rice & maize and its potential applications.
UNIT IV: Forensic Medicine
Criminal investigation (personal identification), Immigration, Paternity dispute,Identification of missing children, bodies found in plane crash, road accidents etc.;Varietal identification of plants.
UNIT V: Molecular Diagnostics
Importance and advantages, Hybridization based methods, Reverse hybridization
methods, Diagnostics based on DNA chips and Micro-arrays, PCR based methods,
Diagnosis of Cystic fibrosis by multiplex PCR , Detection of Thalassemia
mutation using ARMS-PCR, Detection of Fragile X syndrome by FMR-1 gene
trinucleotide repeat analysis, To distinguish patient and donor cells as different
using hypervariable tandem repeat polymorphic DNA markers, Identification of
bacterial species based on the sequences of their 16S ribosomal RNA genes.
Nucleic acid amplification-End-point PCR Qualitative. Real time PCR Qualitative
and Quantitative. SNP based detection: Ligation assay by probe ligation and
amplification (e. g. MLPA). Sequencing based detection.
RECOMMENDED BOOKS
1. Comprehensive Biotechnology by Moo-Young, Murray2. Plant biotechnology In Agriculture: K. Lindsey and M.G.K. Jones (1990), 3. Agricultural Biotechnology by Arie Altman. Marcel Dekker, Inc., 270 Madison Avenue, New. York, USA. 1998. 770 pp 4. Transgenic Plants Vol. 1 & 2 : S-d. Kung and R. Wu (1993), Academic 5. Nano Materials by A k Bandyopadhyay, New age International Publisher6. Plant Cell Culture, Advances in Biochemical Engineering and 7. Human Molecular Genetics. Strachan & Read. 3rd Edition.
8. Genetic Engineering and its applications. (2004) 2/e, Joshi. P: Agrobios, 9. Gene Cloning and DNA analysis: An introduction, (2006) T. A. Brown, 10.Principles of Gene Manipulation; S. B. Primrose, R. M. Twyman & R. W.
old; Blackwell Science, 6th Edition (2001).
PRACTICALS
1. DNA Amplification using PCR2. Cloning of genomic DNA or PCR product in the host bacterium3. Southern blotting4. Multiporation5. Cell fusion by chemical methods PRACTICAL WORK
Note- Any 3 practical’s will be conducted from the above list. Each practical will
be conducted in a group of five students. The practical examination shall consist of
performing an experiment based on the practical work done during the course, the
record of the experiments submitted by the candidate and viva-voce based on the
syllabus
NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to the question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of @ 04 hrs of a topic. The question be a full question of 16/18 marks or have sub-questions to make a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / LEGAL & ETHICAL ASPECTES IN BIOTECHNOLOGY &
INTELLECTUAL PROPERTY RIGHT
Lecture: h / week = 4; Theory paper = 100 marks; Duration: 3h UNIT I: Basic Concepts Of Intellectual Property
Introduction to intellectual property rights, Intellectual property laws, TradeRelated Aspects of Intellectual Property Rights, Forms of IPR like patent designand copyright.
UNIT II: Patents
Introduction to patent law and conditions for patentability, Procedure for obtainingpatents, Rights of a patentee, Patent infringements, Biotechnology patents andpatents on computer programs, Patents from an international perspective.
UNIT III: Copyright
Registration procedure and copyright authorities, Assignment and transfer ofcopyright, Infringement and exceptions to infringement, Software copyright.
UNIT IV: Designs
Introduction to the law on Industrial Designs, Registration and piracy, Internationalperspective, Registration, commercial exploitation and infringement, Patentdatabase and use of the information UNIT V: IPR Laws
Rights/protection, Indian and foreign laws, procurement infringement or violation,remedies against infringement- civil and criminal, Indian Patent Act 2005 andTRIPS.
UNIT VI: Ethics In Patenting
Major changes in Indian Patent system, post TRIPS effects, Contents of patentspecification and the procedure for patents- (a) Obtaining patents (b) Geographicalindication (c) WTO, Detailed information on patenting biological products, Plantbreeders’ and farmers’ rights, Biodiversity, Budapest treaty, Appropriate casestudies, Case studies- PCR, oncomouse.
UNIT VII: Regulatory Aspects Pertaining Pharmaceuticals
Patenting, Role and remit of regulatory authorites-FDA, investigational new drugapplication, European authorities, the centralized procedure, world harmonizationof drug approval RECOMMENDED BOOKS
1. Entrepreneurship: New Venture Creation, David H. Holt2. Patterns of Entrepreneurship: Jack M. Kaplan3. Entrepreneurship and Small Business Management: C.B. Gupta, S.S.
4. F. B. Rudolph, Biotechnology: Science engineering and ethical challenges 5. P. Grubb, Patents for chemicals, pharmaceuticals and biotechnology.
NOTE
For paper setter(s) for setting of question paper(s) for theory examination to be
conducted by University:
1. Weightage to question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of a topic.
The question be a full question of 16/18 marks or have sub-questions tomake a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / ELECTIVE I
5.1 PLANT TISSUE CULTURE AND PLANT BIOTECHNOLOGY
Lecture: h / week- 4; Theory paper = 100 marks; Duration: 3h UNIT I: Plant Tissue Culture
Introduction, History and concept of cellular totipotency; Morphogenesis; Cultureconditions, medium & its constituents; Method of Sterilization UNIT II: Applications Of Plant Tissue Culture- I
Callus culture; Cell Suspension culture; Somaclonal variation; Secondarymetabolite production from plant cell cultures.
UNIT III: Applications Of Plant Tissue Culture-II
micropropagation; Embryo culture, embryo rescue, meristem culture, Antherculture & Ovary culture; Protoplast Culture and Somatic hybridization; Somaticembryogenesis and Synthetic seeds.
UNIT IV: Methods Of Genetic Engineering In Plants
Agrobacterium tumefaciens mediated genetic transformation; Methods of genetransfer.
UNIT V: Genetic Manipulation In Plants For The Following Traits
Herbicide resistance. Male sterility. Virus resistance, resistance towards fungalpathogens. Insect resistance. Genetic engineering of plants for improvement ofcrop yield and quality. Genetic engineering for introducing abiotic stress tolerancein plants. Genetic engineering of nitrogen fixing bacteria; nif genes and it control.
UNIT VI: Molecular Farming
Production of modified carbohydrates, oil, recombinant proteins and vaccines inplants; Molecular markers in crop improvement-Detection of genetic diversity,Marker assisted selection and Breeding.
RECOMMENDED BOOKS
1. Biotechnology: Fundamentals & Applications by S.S.Purohit, Agrobios Ltd.
2. Trends in Plant Tissue Culture & Biotechnology; L K Pareek and P L Swarnkar; Published by AgroBios India (2002).
3. Plant Tissue Culture- Theories and Practices; S. S. Bhojwani and M K Rajdhan; Published by Elsevier, (1996).
4. Methods in Plant Tissue Culture; U Kumar; AgroBios India, (2003).
5. Plant Cell Tissue and Organ Culture Fundamental Methods; O L Gamborge; Published by Narosa, New Delhi (2004).
6. Plant Biotechnology: The genetic manipulation of plants; A. Slater, N.
Scott, M. Fowler; Published by Oxford University press, New York (2003).
7. Transgenic Plants; V. Ranjan, Agrobios NOTE
For paper setter (s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to the question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of @ 04 hrs of a topic. The question be a full question of 16/18 marks or have sub-questions to make a full question of 16/18 marks.
5. There are a minimum of one and a maximum of two questions on each topic 5.2 BIOMATERIALS AND TISSUE ENGINEERING
Theory: h/week – 4; Paper: 100 marks; Duration: 3 h UNIT I: Bioactive Materials And Processing
Calcium phosphate ceramics, bioactive glasses and glass-ceramic, bioactivecoatings and bioactive composites.
UNIT II: Biocompatibility Of Materials
Host responses to materials, biological performance of materials, biocompatibilityassessment, protein adsorption and surface modificatations UNIT III: Biocomposite Materials For Biotechnology
Titanium phosphates, porous-glass ceramic: immobilized enzymes, withbacteriostatic activity, with an integrated skeleton.
UNIT IV: Biotechnology Applications Of Inorganic Glasses
Nature of inorganic glasses, why inorganic glasses? Properties pertaining tobiotechnology and biomedicine UNIT V: Scope Of Tissue Engineering
Scaffold, absorbable polymers, pore creation in biomaterials, special scaffolds,surface modification, cell expansion and differentiation, growth factors.
UNIT VI: Tissue Engineering
Approaches and cells used for tissue engineering, biomaterials used for tissueengineering UNIT VII: Applications Of Tissue Engineering
Skin, bone, peripheral nervous system, heart valve.
RECOMMENDED BOOKS
1. Biomaterials and tissue engineering, Prof. Donglu Shi, Springer, New York.
2. Biomaterials for tissue engineering applications a review of the past and future trends, Jason A. Burdick and Roberts L. Mauck, Springer, New York.
3. Tissue engineering: Fundamentals and application, Yoshito Ikada, Elsevier, 4. Biomaterials Science: An Introduction to Materials in Medicine Buddy D.
Ratner, Frederick J. Schoen, Allan S. Hoffman, Jack E. Lemons.
5. Hench L L Ethridgc E.C. Biomaterials, an interfacial approach, Academic press 1982 Bronzino J D, The biomedical engineering handbook CRC Press.
NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to the question to be asked is based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions are of maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of 04 hrs of a topic. The question be a full question of 16/18 marks or have sub-questionsto make a full question of 16/18 marks.
5. There are a minimum of one and a maximum of two questions on each topic 5.3 NANOBIOTECHNOLOGY
Lecture 4 h / week; Theory paper = 100 marks; Duration: 3h UNIT I: Introduction To Nanobiotechnology
Nano: size and dimension; Nanoscience and Nanotechnology, uses and future ofnanotechnology, potential hazards of nanobiotechnology and safety and publicpolicy issues pertaining to the same UNIT II: Methods Of Measuring Properties Of Nanomaterials
UNIT III: Properties Of Nanomaterials
Metal nano clusters, semiconductors, rare gas and molecular clusters, methods ofsynthesis UNIT II: Nanomaterials
Definition, Types-Nanoparticles, Nanowires, Thin Films, Multilayers andNanocomposites. Effect of size on physico-chemical properties. Applications various fields, Use of nanomaterials for technology development in the fields ofMaterials science, Life sciences with examples.
UNIT III: Nanobiotechnology
Introduction, Definition. Applications of artificially synthesized nanostructures anduse of biomolecules as nanostructures for applications; Synthesis ofnanostructures-Physical methods- CVD, Sputtering, Pyrolysis etc., Chemicalmethods- Sol-gel, Micelle, co-precipitation etc. Bio-based- Using bacteria, fungi,plant extracts, etc., Functionalization of nanostructures for biological applications,Biocompatible coatings and activation of the nanostructures for applications.
UNIT IV: Potential Risks Of Nanobiotechnology
Potential risks, Risk to environment, societal risks, lab safety guidelines forhandling nanomaterials.
UNIT V: Recent Trends In Nanobiotechnology
Development of new instrument for characterization, Current applications ofnanostructures in life sciences, textile industry and fuel cells RECOMMENDED BOOKS
1. Nanotechnology –S. K. Kulkarni – (Capital Publishers –Delhi)2. A handbook on nanotechnology – A. G. Brecket (Dominant Publishers and 3. Nanotechnology: Research and Perspectives - Dr. Sidharth Vaidy (Pearl 4. Biofunctionalization of Nanomaterials – Ed. Challa S. S. R. Kumar (Willey- 5. For recent trends and protocols: see recent published research and review articles from reputed international journals.
6. Nanotechnology, by Dr. Shalini Suri, APH Publishing Corporation.
7. Nanotechnology, by M. Ratner and Daniel Ratner, Pearson Education.
8. Nanobiotechnology fundamentals and applications by Manasi Karkare, Published by I K International publishing house, New Delhi.
9. Introduction to nanobiotechnology by Charles P. Poole Jr and Frank J.
Owens, Published by John Wiley and Sons, USA.
NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to the question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of a topic.
The question be a full question of 16/18 marks or have sub-questions tomake a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / PROJECT PART - I
The project work is to impart training in biotechnology engineering. Theknowledge gained by studying various subjects separately is utilized for a singletask. The project trains to co-ordinate the knowledge of biotechnology principlesassimilated over the period of course study and about forty subjects. This is anexercise in literature survey, report writing and team work. The project reportreflects on the devotion of students towards work and single mindedness approach.
A group of 2 student or single student will have to work on a topic assigned tothem or him/her. One staff member will supervise the work of the students. Theproject work may involve experimental/ theoretical/computational work.
A preliminary report is to be submitted containing the details of literature survey,data collected, and outlines of project.
Inline with the project topic, students will be given a research and or review paperon which he or she has to deliver a power point presentation of 5-8 min in front ofthe faculty of the department. 20% term work marks will be based on thispresentation.
The term work will be assessed by two university approved internal examinersappointed by the Principal of the college, one of whom will be his guide & theother a staff member of the concerned branch.
PROCESS CONTROL AND SEPARATION
Theory: h/week - 4; Paper: 100 Marks; Duration: 3 h Practical: h/week - 2; Practical Examination: 25 marks, Term Work: 25 marks UNIT I: Membrane Separation
Classification of separation techniques, Membrane separations, Definition of amembrane, Criteria of membrane separation processes, Types of membranes,Advantages of membrane separation processes over conventional separationtechniques, Industrial Applications, Micro filtration, Ultra filtration, ReverseOsmosis, Piezodialysis, Electro dialysis, Pervaporation, Carrier mediated transport-liquid membranes, Membrane contactors, and industrial applications of allprocesses.
UNIT II: Control System
Introduction to control system, parts of control systems, lags inherent ininstruments and process. Mathematical modeling of bio-technology/chemicalprocess, state variables and state equations for a chemical/bio-tech. process. Theinput out put model, Linerization of non-linear systems, solution of lineardifferential equation using laplas transform. Feedback and feed forward control.
Block diagram and development of block diagram.
UNIT III: Dynamic Behavior Of First Order System
First order system and their transfer functions. Dynamic behavior of first ordersystem, pure capacitive process, first order system with variable time constantgains. Response of first order system in series, interacting and non-interactingsystems, Derivation of transport equation.
UNIT IV: Dynamic Behavior Of Second Order System
Second order system and their transfer functions. Under damped and over dampedand critically damped systems, transportation lag. Dynamic behavior of highersystems. Derivation of transport equations.
UNIT V: Feed Back Control
Introduction to feed back control, control elements. Dynamic behavior of feed backcontrol processes. Closed loop transfer function control action, block diagram andapplication of control system for chemical reactors, distillation column adsorptioncolumn, heat exchange equipment like DPHE, S & THE and evaporator.
UNIT VI: Controllers
Proportionate, proportionate Derivative, proportionate integral and proportionateIntegral Derivative. Design of feed back controller: performance criteria, selectionof type of controller. Tuning of feed back controller. Stability analysis by Routcriteria and Root locus analysis.
UNIT VII: Frequency Response
Frequency response analysis of linear processes; Bode’s diagram, Nyquist plot.
Design of feed back control system using frequency response techniques, Bode’sstability diagram, criteria gain and phase margin Ziegler-nichols techniqueNyquest stability criteria. Advance control strategies. Cascade control, feedforward control, ratio control, selective control, split range control, adaptive andinferential control Introduction to analogue, digital computer and DCS.
RECOMMENDED BOOKS
1. Chemical process control: An Introduction to theory and practice, George Stephanopoulos, prentice Hall Of India.
2. Process System Analysis and Control, D. R Coughnour, et al McGraw Hill 3. Process control, Peter Harriot Tata McGraw Hill Publication.
4. Principle of Industrial Instrumentation, D Patranabis, McGraw Hill SUGGESTED LIST OF EXPERIMENTS
1. Thermocouple/Thermometer2. Manometer3. Liquid level system4. Thermal system5. Pneumatic system6. Pneumatic controller PRACTICAL WORK
Note-Minimum five experiments, based on the syllabus, should be conducted
during the course and record (journal) for the same shall be submitted. The
practical examination shall consist of performing an experiment based on the
practical work done during the course, the record of experiments submitted by the
candidate and viva-voce based on the syllabus.
NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to the question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of @ 04 hrs of a topic. The question be a full question of 16/18 marks or have sub-questions to make a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / PROTEIN ENGINEERING
Lectures: h/week - 4; Theory: 100 marks; Duration: 3 h UNIT I: Basics Of Proteins
Overview regarding biosynthetic and degradative pathways of proteins,Conformation studies of different proteins, Specific conformation of enzymesconsidering position of active sites. Effect of amino acids on structure of proteins,Energy status of a protein molecule, structure and function relation of enzymes.
UNIT II: Structural Elucidation Of Proteins
Physical methods such as X-ray, Site directed mutagenesis for specific proteinfunction.2D and 3D gel electrophoresis, isoelectric focusing UNIT III: Designing Of Peptide And Enzyme Engineering
Basic concepts for designing a new protein / enzyme molecule. Specific examplesof enzyme engineering, Tryesyl T RNA Synthatase. Dihydrofolate reductase,Subtilisin. Chemical synthesis of specific amino acid sequence, Carboxylic acidspecific immobilization, amino group protection by acetylation.
UNIT IV: Protein Modification
Chemical modification in the homologous and heterologous proteins producedfrom prokaryotes: Phosphorylation glycosylation, methylation, formylationmethioniation and demethionation.
UNIT V: Applications Of Protein Engineering
Production of antibiotics, antibody molecules UNIT VI: Advancement In Protein Engineering
Peptide mass finger priniting: MS-ESI-TOF and MALDI-TOF, crystallography fordetermination of protein structure RECOMMENDED BOOKS
1. Klaus Demobowsky, Novel Therapeutic Proteins: Wiley Publications.
2. Messer- schmidt, Hndbook of Metaloproteins – Wiley Publications.
3. Ronald Kellner et al., Microcharacterisation of proteins, 2nd ed. Wiley, 4. Susane Brakmann, Directed Molecular Evolution of Proteins- Wiley 5. Walsh, Protein Biotechnology and biochemistry, 2nd ed., Wiley Publications6. Westermeier – Proteomics in Practice- Wiley Publications.
7. Buchanan B.B. Grussem. W. and Jones. R.L. 2000. Biochemistry and 8. Biology of Plants. American Society of Plant Physiologists, Maryland, USA.
9. Protein engineering: applications in science, medicine, and industry by
10.Protein engineering by Jeffrey L. Cleland, Charles S. Craik - 1996 - 518 11.Protein engineering by Morio Ikehara - 1990 - 355 pages12.Proteins by Thomas E. Creighton - 1993 - 507 pages13.Protein engineering by Peter C. E. Moody, Anthony J. Wilkinson, Tony 14.Protein Engineering Handbook, Volume 1 Stefan Lutz, Stefan Lutz (Prof.), PRACTICALS
1. Tube gel electrophoresis2. Paper electrophoresis3. Determination of isoelectric point4. SDS PAGE5. NATIVE PAGE6. 2D gel electrophoresis PRACTICAL WORK
Note-Minimum four experiments, based on the syllabus, should be conducted
during the course and record (journal) for the same shall be submitted. The
practical examination shall consist of performing an experiment based on the
practical work done during the course, the record of experiments submitted by the
candidate and viva-voce based on the syllabus.
NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University
1. Weightage to the question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of a topic.
The question be a full question of 16/18 marks or have sub-questions tomake a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / PHARMACEUTICAL BIOTECHNOLOGY
Lecture: h / week - 4; Theory paper = 100 marks; Duration: 3h UNIT I: Introduction To Pharmaceutical Biotechnology
Pharmaceutical biotechnology and its industrial applications, prokaryotic andeukaryotic cell in biotech production, biopharmaceuticals expresses in plants UNIT II: Therapeutic Proteins
Pharmacokinetics and pharmacodynamics of biotech drugs, formulation of biotechproducts, Toxicity studies, Clinical trials peptide and protein drugs and recentadvances-gene therapy, antisense.
UNIT III: Vaccine Production
Scientific, technical and economical aspects, production of DNA vaccines,characterization and bio analytical aspects of recombinant proteins.
UNIT IV: Product Analysis
PROTEIN based contaminants, detection of protein based impurities,immunological approaches, endotoxin and pyrogenic contaminants, miscellaneouscontaminants, Validation studies.
UNIT V: Case Study Of Industrially Important Metabolites
Study of following metabolites from biotechnology perspective- cytokines, growthfactors, therapeutic hormones, recombinant blood products and therapeuticenzymes.
UNIT VII: Drug Development Process
History of biopharmaceuticals, Drug development process, preclinical studies,pharmacokinetics and pharmacodynamics, toxicity studies, delivery ofpharmaceuticals and role and remit of regulatory authorities.
RECOMMENDED BOOKS
1. Proteins: Biochemistry and Biotechnology - Gary Walsh2. Pharmaceutical biotechnology concepts and applications – Gary Walsh, 3. Pharmaceutical biotechnology: Drug discovery and clinical applications, Oliver Kayser and Rainer H Muller, Wiley.
4. Foye’s Principles of Medicinal Chemistry –William David A. and Lemke 5. Pharmaceutical Biotechnology, 2nd ed. by Crommelin D.J.A. & Sindelar R.
NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to the question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of a topic.
The question be a full question of 16/18 marks or have sub-questions tomake a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / 10 ELECTIVE II
10.1 ENVIRONMENTAL ENGINEERING
Lecture: h / week – 04; Theory paper = 100 marks; Duration: 3h Practical: h / week 02; Term work = 25 marks UNIT I: Environmental Biotechnology: Vision And Development
Biodiversity, Ecology, Pollution control, Agriculture, Bio-energy UNIT II: Biodiversity And Ecology
Biosphere, Ecosystem, Natural cycles (Nitrogen, Carbon, Phosphorus, Sulfur),Energy flow.
UNIT III: Environmental Pollution And Control
Water pollution, Sources of pollutants, Waste water processing, Advanced wastewater treatment in different industries (Brewery, Petroleum, paper and pulp); Soilpollution, Sources of pollutants, Bioremediation-In-situ (intrinsic, geneticallyengineered), Ex-situ (solid phase treatment slurry phase treatment, vermicomposting); Air pollution-Sources of pollutants, Removal and control; Hazardouswaste management - Physical treatment, Chemical treatment, Biological treatment,Xenobiotic compounds and their degradation. Characteristics of xenobioticcompounds, Examples of xenobiotic compounds, Mechanism of degradation.
UNIT IV: Environmental Impact Assessment (EIA)
Need and objectives of EIA, Factors involved in EIA, Environmental attributes,Methods of EIA - Ad-hoc, Overlay, Network, Matrix, Checklist, Cost benefit,limitations and problems of EIA UNIT V: Global Warming And Green House Effect
Reasons for global warming, Technique of Green house effect, Applications ofGreen house effect.
UNIT VI: Agricultural Biotechnology
Pesticides and Insecticides-types, mode of action and applications; Herbicides-types, mode of action and applications; Fungicides- types, mode of action andapplications; Biopesticides-B. thuringensis- mode of action and applications; Viralpesticides-example, mode of action and applications, Neem pesticides mode ofaction and applications; Biofertilizers - Nitrogen fixing organisms their examples,mode of action and applications; Phosphorus solublizing organisms their example,mode of action and applications; Genetically modified organisms-need,application, stress tolerant plants.
UNIT VII: Bio-Energy
Renewable sources of energy; Biomass as a source of energy; Energy crops-Production and applications.
UNIT VIII: Integrated Environmental Management (Overview)
RECOMMENDED BOOKS
1. Environmental Biotechnology; M. H. Fulekar; Oxford & IBH Publishing 2. Environmental Biotechnology; Dilipkumar Markandey; APH Publishing 3. Environmental Biotechnology: theory and applications; G. Evans & J.
Furlong; Wiley & Sons Pvt. Ltd; 1st edition 4. Textbook of Environmental Engineering; P. Venugopal Rao; PHI Pvt Ltd; 5. Environmental Biotechnology; Alan Sragg; Oxford University Press; 2nd 6. Biotechnology; B. D. Singh; Kalyani Publications; 1st edition7. Water and Waste Water Technology; Hammer & Hammer Jr.; PHI Pvt Ltd, 8. Green House Technology for controlled Environment; G N Tiwary; Narosa NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University
1. Weight age to the question to be asked be based on number of teaching hours allotted to each topic / unit.
2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of 04 hrs of a topic. The question be a full question of 16/18 marks or have sub-questionsto make a full question of 16 / 18 marks.
5. There be a minimum of one and a maximum of two question on each topic / 10.2 ANIMAL CELL SCIENCE AND TECHNOLOGY
Lecture: h / week - 4; Theory paper = 100 marks; Duration: 3h Practical: h / week - 4; Term work = 25 marks; Practical Exam: 25 marks UNIT I: Introduction To Animal Cell Culture
Historical Background. In-vitro and in-vivo cell metabolism. In-vitro growthpromoting factors: Physiological and nutritional factors, Growth factors [EGF,FGF, PDGF, NGF, IGF, CSF, Erythropoietin], Hormones UNIT II: Prerequisites Of Cell Culture
Balanced salt solution. Media for culturing cells and tissues – Natural, Artificial.
Sterilization procedures. Bioethical issues related to animal biotechnology UNIT III: Establishment Of Cultures And Their Maintenance
Initiation of primary cultures from anchorage dependant and non- anchoragedependant cells. outine maintenance of cultured cells. Detection and prevention ofcontamination. Established cell lines- transformation. Commonly used cell lines- origin and characteristics. OrganCultures UNIT IV: Analysis Of Cell Growth
Growth kinetics of primary and established cultures. Characterization of cell lines.
Measurement of cell death in-vitro UNIT V: Specialized Culture Techniques
In-vitro generation of lymphocytes from embryonic stem cells. Isolation andculture of skeletal muscle myofibres as means to analyze satellite cells. ArtificialSkin. Culture of human and rat Schwann cells. Long-term preservation of animalcells/ cell lines.
UNIT VI: Scaling Up Of Animal Cells
Bioreactors for monolayer cultures. Bioreactors for suspension cultures.
Immobilized cultures-Immurement cultures, Entrapment cultures UNIT VII: Applications Of Animal Biotechnology
Use of cell culture for production of a regulatory protein. Use of cell culture forproduction of a hormone (e.g. Insulin). Use of cell culture for production ofvaccines. Cell hybridization and human hybridization. Use of cell culture in drugtargeting and drug toxicity analysis. Transplantation of cultured cells.
UNIT VIII: Stem Cell Biotechnology
Types and characteristics of stem cells. Culture conditions/equipments essential forembryonic stem cell cultures. Use of stem cell cultures for treatment of geneticdisorders UNIT IX: Transgenic Animals
Methods of transgenesis. Vectors involved. Biopharmaceutical applications oftransgenic animals.
RECOMMENDED BOOKS
1. C. Helgasson; Basic cell culture protocols, 3rd edition, Human press2. E. D. Rang, H.P. Dale, M.M. Ritter; Pharmacology, 5th edition3. J. Mather and d. Barnes; Animal cell culture methods, Elsevier, vol 574. J.R.W. Masters; Animal Cell Culture- A practical approach, Oxford 5. J. Paul Basic Protocols in cell and tissue culture6. M. Butler; Animal cell technology-Principles and products, Open University 7. M. Butler and M. Dawson, Cell culture lab fax, Bios scientific Pvt. Ltd.
8. M. Cylnes; Animal cell culture techniques, Springer Verlag9. M.M. Young; Animal Biotechnology, Pergamon press, Oxford10.N. Jenkins; Animal cell biotechnology-Methods and protocols, Human Press11.R.I. Freshney; Culture of animal cells: A manual of basic techniques, John NOTE
For paper setter(s) for setting of question paper(s) for the theory examination to be
conducted by University:
1. Weightage to question to be asked be based on number of teaching hours 2. Total of 05 (five) questions maximum, be asked per section of the paper, out of which students are expected to answer / solve any three questions.
3. Questions be of a maximum 16 / 18 marks each, to add up to maximum 50 4. Questions of maximum 16 / 18 marks are expected for teaching of @ 04 hrs of a topic. The question be a full question of 16/18 marks or have sub-questions to make a full question of 16/18 marks.
5. There be a minimum of one and a maximum of two question on each topic / 10.3 FOOD BIOTECHNOLOGY
Theory: h/week - 4; Paper: 100 marks; Duration: 3 h SECTION A: FOOD BIOTECHNOLOGY
UNIT I: Overview Of Food Biotechnology
Biotechnology in relation to the food industry, Food processing, generalengineering aspects and processing methods. Preliminary processing methods,conversion and preservation operations. Nutritional value of food, spoilage of food,Microorganisms in water, milk, vegetables, fresh meats and poultry, processedmeats, seafood’s, fermented dairy products and miscellaneous food products.
UNIT II: Food Preservation
Introduction to Physical and chemicals methods of food preservation: Principlesand methods of food preservation- Refrigeration, Freezing, heating, dehydration,drying, canning, extrusion Cooking, hydrostatic pressure cooking, dielectricheating, microwave processing, aseptic Processing juices and concentrates,membrane technology, additives, irradiation. Storage of food, modified atmospherepackaging.
UNIT III: Microbial Technology In Production Of Different Food Products
Technologies used for microbial production of food ingredients, biotechnology ofmicrobial polysaccharides in food, microbial biotechnology of food flavorproduction, microbial production of oils and fats, food applications of algae,butanol production from agricultural biomass SECTION B: FUNCTIONAL FOODS (NUTRACEUTICALS)
UNIT IV: Functional Foods
Food or medicine? Concept of per and probiotics, use of nutraceuticalsupplements, safety, major Nutraceuticals and their applications.
Metabolism,
Bioavailability,
Pharmacokinetics
Nutraceuticals
UNIT VI: Nutraceutical And Cardio Vascular Health
Black and green tea, soy, essential fatty acids, coenzyme Q10, Lycopene,octacosanol, melatonin, Resveratrol, luetin.
UNIT VII: Nutraceutical And Cancer Prevention
Tea, lycopene, flax seed, PUFA, coenzyme Q10 11 PROJECT PART - II
A group of 2 students or a single student who have (has) been assigned a topic inProject-I is expected to complete the details of the project. A group of 2 students ora single student will have to submit a detailed typed & bound report of the workdone, in Project-I and Project-II combined together. The practical examinationshall consist of a viva-voce based on the project work completed in Part-I and Part-II by the candidate.
It will be mandatory for the students to present their project work in any national orinternational conference or even they can submit their manuscript based on theirproject work to any national or international journal or conference proceeding. Thestudents must attach a Xerox copy of the certificate of merit or participation intheir project report.

Source: http://jnec.org/syllabus/btech/B.E._Biotechology.pdf