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Preparation and in vitro evaluation of tretinoin nano-emulsion system
Mahsa Sabouri 1, Effat Sadat Farboud 2, Mansour Nasiri Kashani 3 Zahra Jafari Azar 1, Saman Ahmad Nasrollahi 3٭ 1. Department of Pharmaceutics, Islamic Azad University of Pharmaceutical Sciences, Tehran, Iran 2. Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, 3. Center for Research & Training in Skin Diseases & Leprosy, Tehran University of Medical Sciences, Abstract Summary
homogenizer. The pressure varied in the range of The purpose of this study is to construct 300-1000 bar. The Z-average and zeta potential was analyzed by Malvern Zetasizer ZEN 3600 formulation with skin targeting for topical delivery of tretinoin which is important in microscopy (TEM). Accelerated stability tests reducing side effects of tretinoin in target site. (Z-average, centrifuge, viscosity and …) were Nanoemulsions consist in very fine emulsions, done at 40 C and 75% humidity for 6 months. with a droplet diameter smaller than 200nm. They can be prepared by using a high shear and Results and Discussion
high pressure devices, which allows a better The property of the particles depends on the control of the droplet size and a large choice of amount of surfactant, production pressure and the number of homogenization cycles. The best formulation indicates z-average of 112.5 nm, Introduction
Pd.I of 0.137 and zeta potential of -45.95 mv. Tretinoin has been increasingly at the center This formulation is stable for 6 months during of attention because of its efficiency in topical healing of acne vulgaris, ichtyosis, psoriasis, and neoplasia. Leads to local irritation such as erythema and flaking at the application site and increased weakness to sunlight it is observed limited tolerability by consumers. In this study we developed nano-emulsions as an alternative carrier system to usual emulsions and gels in order to make the possibility of controlled drug release, drug targeting, and reduce the local side effects of tretinoin. Figure 1. TEM image of best formulation. Materials and Methods
We formulated NE with nonionic surfactant Conclusion
and high pressure homogenization technique (HPH). For all formulations the lipid phase was fabricated by HPH method is stable. Also this method has a good potential to scale up simply in surfactant at 75 C and a premix was formed by pharmaceutical industries. We suggest this novel homogenizing in an IKA Ultra Turrax T25 high- drug delivery system for topical delivery of speed stirrer. The premix was passed through an tretinoin with more advantages than conventional References
1. Lipid nanoparticles (SLN, NLC) in
cosmetic and pharmaceutical dermal products.
2009 (366): 170–184.
2. Saman Ahmad Nasrollahi, Alireza Abbasian,
Effat Sadat Farboud: Invitro comparison of
simple tretinoin-cream and cream loaded with
tretinoin-SLN. J. Pharm. Tech. Drug Res., 2013
(2): 1-7.
3. Hamid Reza Kelidari, Jafar Akbari, Majid
Saeedi: Application and Characterization of
Solid Lipid Nanoparticles and Nanostructured
Lipid Carriers as Drug Delivery Systems. J.
Mazand. Univ. Med. Sci., 2013 (23): 387-403.

Source: http://crsi.ir/wp-content/uploads/2013/11/mahsa-sabouri.pdf

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